Coding

Part:BBa_K209493:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-01)

AarI C-D part, KIFC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1224
    Illegal PstI site found at 181
    Illegal PstI site found at 901
    Illegal PstI site found at 934
    Illegal PstI site found at 1141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1224
    Illegal NheI site found at 687
    Illegal PstI site found at 181
    Illegal PstI site found at 901
    Illegal PstI site found at 934
    Illegal PstI site found at 1141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1224
    Illegal BglII site found at 659
    Illegal BamHI site found at 43
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1224
    Illegal PstI site found at 181
    Illegal PstI site found at 901
    Illegal PstI site found at 934
    Illegal PstI site found at 1141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1224
    Illegal PstI site found at 181
    Illegal PstI site found at 901
    Illegal PstI site found at 934
    Illegal PstI site found at 1141
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 131
    Illegal BsaI.rc site found at 548
    Illegal BsaI.rc site found at 670
    Illegal BsaI.rc site found at 680


Design Notes

Cloned into pCR2.1.

Source

References